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Research Cores
X-ray Crystallography core

 Project Director, PI  , UGA
 UGA Project Director  , UGA
   Shu-Huey Chang,UGA
   Hua Zhang, UGA
 UAB Project Director, Co-PI  , UAB
 Project Director, Co-PI  , UAB
   , UAB
   , UAB
 GSU Project Director  , GSU
   , GSU
   Yen Fang Wang, GSU
   Bhuuaneshwari Mahalingham, GSU
   John Petock, GSU
 UAH Project Director  , UAH
   Joseph Ng, UAB
   Liqing Chen, UAB
Crystallization Technology Director  , UAB
   Lisa Nagy, UAB
   Hongli Chen, UAB
   Analiza, Inc.
 Phasing Methodology Director  , UGA
   Zhengqing(Albert) Fu, UGA
   Zhijie(James) Liu, UGA
 Beamline Automation Director  , UGA
   Gerd Rosenbaum, SERCAT
   John Chrzas, SERCAT
   Jim Fait, SERCAT
   Zhongmin Jin, SERCAT
 Software Development Director  , UGA
   Lirong Chen, UGA
   George Wu, UGA
 Project Director  Robert Harrison, GSU

The XRC Core consist of four modules:

A Robotic Crystallization Module, in which microgram quantities of protein (bar coded) will be screened, using robotics and mirco-arrays, against 50-100 sets of conditions known to produce protein crystals. A high throughput robotic crystallization system, capable of screening 1000 conditions per hour using less than 300 micrograms of protein (10 mg/ml), is currently under development at the University of Alabama at Birmingham. (more details)

A Data Collection Module, where the necessary X-ray diffraction data needed to determine structure are collected on the protein crystals in X-ray diffraction laboratories at UGA. UAB, UAH, GSU and via robotics and tele-presence at the Southeast Regional Collaborative Access Team's (SER-CAT) synchrotron beamlines at the Advanced Photon Source, Argonne National Laboratory, outside of Chicago, IL. SECSG partners have contributed over $4 million towards the constriction of the SER-CAT beamlines. The first beamline is expected to be fully operational in early 2002. The second beamline will begin construction in June 2001 and will be operational in 2003.

A Phase Calculation Module
, where University of Georgia partners are developing rapid and cost effective phasing methods, including direct determination of protein structure using single-wavelength data.

A Computer Graphics Module, where the protein sequence is fitted into the electron density maps generated from the X-ray diffraction data. Once the sequence has been fitted, the structure will be refined against the X-ray data and stereochemistry of the structure to give the best agreement with the experimental data.

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